ABSTRACT
Background and Objectives: Muscular atrophy is one of the most common complications of diabetes. In such cases, protein degradation isincreased and protein synthesisis decreased. MuRF1 is an E3 ubiquitin ligase which has been identified as a mediator of skeletal muscle wasting in various skeletal muscle atrophy models, and its expression is upregulated in atrophy. Exercise training has been suggested as one of the treatment strategies for muscular atrophy. The aim of this study is to investigate the effects of 4 weeks of resistance training on MuRF1 gene expression in muscular atrophy in streptozotocin-diabetic wistar rats
Material and Methods: Thirty six male Wister rats [288 +/- 22 g] were randomly divided into four groups: nondiabetic control, non-diabetic trained, diabetic control and diabetic trained. The exercise groups were subjected to a resistance training program using a ladder [3 days/wk, for 4 wk]. MuRF1 mRNA level was measured in Flexor Hallucis Longus muscle using Real-Time PCR. The results were studied by statistical methods
Results: MuRF1 gene expression was increased in rats with diabetes [p=0.001]; resistance training diminished the skeletal muscle wasting in diabetic rats [p=0.002] by inhibiting MuRF1 gene expression
Conclusion: This study indicates that short term resistance training can overcome diabetes-induced atrophy in rats. Whether this kind of training might be a good way for countering atrophy in other diseases with similar catabolic situation to diabetes remains to be elucidated
ABSTRACT
Backgrounds and Objectives: Omentin is a member of adipokines secreted from visceral adipose tissue which increaseS insulin sensitivity. The aim of the present study was to investigate the effect of endurance and resistance training on plasma omentin-1 levels in male rats
Materials and Methods: In this experimental study, twenty-four Wistar male rats with average weight of 220 +/- 20 gr were acclimatized with lab condition and then randomly divided into three groups of Control [C], Resistance training [RT] and Endurance training [ET]. Endurance training group underwent an endurance training program for eight weeks [five bouts per week of treadmill running each 60min with 20 m/min]. Resistance training group was subjected to a three-day per week resistance training program consisting of climbing a ladder carrying a load suspended from the tail. At the end of the experimental period, blood samples were taken to assess plasma omentin-1, insulin and glucose levels. Data were analyzed using one-way ANOVA and statistical significance was set at P0>.05
Results: The results showed that endurance training significantly increased plasma omentin-1 levels and decreased plasma insulin and glucose levels while plasma omentin-1 and insulin levels did not change significantly in the resistance group
Conclusion: Endurance training was more effective on omentin-1 levels than resistance training. Either training programs inhibited weight gain observed in the control group. It might be hypothesized omentin-1 contributes to metabolic effects of exercise training